FIG. 7.
Changes in the numbers of cytokine-producing cells during M. avium infection. (A) ELISPOT assays were used to determine the numbers of IL-2- and IFN-γ-producing cells after stimulation in vitro with M. avium. Cells cultured without M. avium had ≤5 cytokine-producing cells/106 spleen cells (data not shown). The values are means and standard deviations based on the data for groups of three mice. The results of one representative experiment of three independent experiments are shown. Symbols: ▪, IL-2-producing cells; □, IL-2 normal cells; •, IFN-γ-producing cells; ○, IFN-γ normal cells. (B) Scatter plot of the number of IFN-γ- and IL-2-producing cells from the same mouse at different times after M. avium infection. Data pooled from three independent experiments are shown.