Fig. 2.
Decreased cell division in SVZ of E14.5 Frs2α2F/2F mice. (A–E) BrdUrd pulse-labeled cells in telencephalon at E12.5 (B and D) and percentage of BrdUrd-labeled cells in 10 randomly selected areas of coronal sections (E) (values are mean ± SD). A and B and C and D are the same sections. Nuclei are labeled with Hoechst 33258 in A and C.(F–P) The number of phosphohistone H3-positive cells are decreased in SVZ but not in the apical side of VZ in Frs2α2F/2F mice. (G, I, K, and M) Coronal sections were immunostained with anti-phosphohistone H3 antibodies. Arrows indicate stained cells in the apical side of VZ. Arrowheads indicate stained cells in SVZ. F and G, H and I, J and K, and L and M are the same sections. (F, H, J, and L) Nuclei were labeled with Hoechst 33258. (N–P) Labeled cells were counted in 10 randomly selected areas of the coronal sections and are reported as mean ± SD per area unit. *, P < 0.05; **, P < 0.01 vs. WT or heterozygous.