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The X chromosome is the source of Penelope siRNA within the female germ line. In 0- to 2-h embryos, Penelope siRNA is found only if mothers possess the 160 X chromosome. In females, the X chromosome drives the expression of Penelope siRNA in the germ line and soma. Females that lack this chromosome do not demonstrate Penelope siRNA in either tissue. Five micrograms of enriched RNA was used per lane.
