FIG. 4.

Quantitative analysis of adhesion of rFimA-MS to epithelial cells. (A) Confocal microscopic analysis of adhesion of rFimA-MS. HEp-2 cells were cultured on LabTekII chamber slides (eight wells; 2 × 10⁴ cells per well) for 2 days at 37°C. rFimA-MS were added to each well and incubated for 4 h. After incubation, the cells were extensively washed and fixed. Images (red, rFimA-MS) were collected and analyzed with a confocal microscope. Magnification, ×400. Bar, 50 μm. (B) Time course of adhesion of rFimA-MS (type I [▪], type II [⧫], type III [•], type IV [▴], or type V [▾]) or BSA-MS (◃) to HGF. The amounts of rFimA-MS and BSA-MS that adhered are expressed as the total areas of the red images in the field of a confocal microscope (magnification, ×400), and at least five fields were analyzed for each experiment. The values are means and standard errors of the means based on three individual experiments. An asterisk indicates that a P value is <0.01.