Figure 9.

Exo70p depletion results in mislocalisation of FIP3 and Rab11 in the furrow. (Top panels) Representative fields of HeLa cells transfected with either scrambled or Exo70 siRNA. At 48 h after transfection, cells were immunostained with anti-FIP3 (green) or anti-Exo70p (red) antibodies. The bright field image at the right shows the presence of the furrow and midbody. (Bottom panels) Representative field of HeLa cells transfected with Exo70p siRNA and immunostained for FIP3 (green) or Rab11 (red) (cf. bright field image at right). The asterisk indicates the position of the midbody. For comparison, a cell transfected with a scrambled siRNA and stained for Rab11 is shown at the left.