Figure 2.

Fz protein abundance and surface localization in S2 cells. (A) Whole-cell protein extracts of S2 cells transfected with fz transgenes probed with a polyclonal antibody directed against the ‘hinge' region of Fz (α-Fz, top panel), an epitope, which Fz2 lacks. When the same samples are probed with an HA antibody, we observed that Fz2^ΔCRD is expressed comparably to Fz^ΔCRD3, which are both expressed at higher levels than Fz^ΔCRD2. The molecular mass in kDa of a known protein standard is indicated. (B) Cell surface localization of Fz protein was assayed in S2 cells transfected with fz transgenes and stained using an antibody directed against the ‘hinge' region of Fz. Fz2 was not tested as it lacks this epitope. The same settings were used for all samples aside from mock-transfected cells where the gain was intentionally set higher to emphasize lack of signal. Staining is specific, as untransfected cells (^*) are not detectable. The scale bar in upper left panel is 10 μm.