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Arm signaling of fz CRD variants in S2 cells. (A) To measure Arm signaling, TCF/LEF-dependent luciferase reporter assays were performed on cells cotransfected with pTub (Mock) or a pTub-fz variant and empty pTub (−) or pTub-wg (+). (B) TCF/LEF-dependent luciferase reporter assays were performed on cells cotransfected with pTub (Mock) or a pTub-fz variant and challenged with plain medium (−) or medium containing purified Wg (+).
