FIG. 2.

IFN-γ in vitro secretion from splenocytes isolated from vaccinated mice that were aerogenically challenged with M. tuberculosis Erdman. Vaccinated mice were sacrificed 7 days (A) or 14 days (B) following aerogenic challenge, and splenocytes were restimulated in vitro using bone marrow macrophages primed with either the CFP mixture (gray boxes) or the recombinant protein mixture (black boxes). The amounts of IFN-γ that were secreted were determined by ELISA. Asterisks indicate that the amount of cytokine secreted is statistically significant compared to the vector control group, as determined by ANOVA (P < 0.05). Please note that the scales in panels A and B are different because the 14-day cytokine responses were substantially larger than the 7-day responses. Error bars, standard errors.