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. 2002 Jan;70(1):62–68. doi: 10.1128/IAI.70.1.62-68.2002

FIG. 1.

FIG. 1.

BT1 gene inactivation in L. donovani. (A) The BT1 locus of L. donovani 1S, along with PstI sites, is shown, as well as the integration of the HYG expression cassette (32), which carries an extra PstI site. (B) Southern blots of L. donovani cells hybridized with a BT1 probe (left) and a HYG probe (right). Sizes of the bands were determined using the 1-kb ladder. Lane 1, L. donovani wild-type cell; lane 2, L. donovani BT1 null mutant; lane 3, L. donovani BT1 null mutant retransfected with a plasmid expressing the BT1 gene.