FIG. 5.

IFN-γ production by splenic T cells in immunized mice. Splenocytes of BALB/c mice immunized with either the L. donovani wild type or the L. donovani BT1 null mutant were isolated at 1, 2, 4, 8, and 12 weeks postinfection, stimulated with L. donovani promastigotes, and tested by ELISA for the production of IFN-γ as described in Materials and Methods. Results are representative of two independent experiments, each containing three replicate wells with pooled splenocytes from five mice.