TABLE 1.
Time course of plasma cytokinesa
| Cytokine | Concn of cytokine (pg/ml) atb:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Control | 1 h | 2 h | 4 h | 8 h | |||||
| L | F | L | F | L | F | L | F | ||
| TNF-α | 0 | 2,800 (447) | 386* (50) | 1,898 (123) | 144* (13) | 588 (67) | 247* (176) | 245 (138) | 358 (144) |
| IFN-γ | 0.2 (0.2) | 1.7 (1.2) | 2.3 (1.9) | 72 (21) | 86 (29) | 989 (165) | 430* (59) | 1,312 (238) | 430* (54) |
| IL-6 | 13 (4) | 4,629 (1,149) | 549* (83) | 20,197 (1,810) | 651* (119) | 9,934 (340) | 290* (22) | 5,558 (860) | 277* (39) |
| IL-10 | 11 (8) | 1,388 (252) | 83* (7) | 1,801 (476) | 98* (17) | 1,061 (194) | 97* (18) | 356 (52) | 75* (17) |
a
Conscious mice were challenged at baseline with an i.v. injection of S. enterica serovar Enteritidis LPS (L) or S. enterica serovar Muenchen flagellin (F), both at 4 mg/kg (100 μg/mouse). A group of five mice not treated with LPS or flagellin was used for control purposes. Mice were killed after 1, 2, 4 and 8 h, and plasma was obtained for the measurement of circulating cytokines. n = 9 to 10 mice/group at each time point.
b
Values are means with standard errors given in parentheses. *, P < 0.05 for LPS versus flagellin. All values in the L and F groups were significantly higher than those in control mice.