FIG. 3.

Transmission electron micrographs of sheep erythrocyte membranes treated with the SphH. Sheep erythrocytes were mixed with 0.1 M NaCl SphH eluate and reacted at 37°C for 20 min. (A) Control sheep erythrocyte mixed with PBS (pH 7.2) or lysate of E. coli harboring pBS vector. (B and C) Sheep erythrocytes treated with SphH eluate. Arrowheads indicate the membrane pores induced by SphH, and arrows indicate the burst membrane regions caused by water influx through the SphH-induced pores by osmotic pressure. Magnification, ×16,000 (A and B) and ×40,000 (C).