FIG. 1.

Expression of leptospiral antigens in E. coli. Several clones obtained from the expression library were transformed as plasmid DNA into E. coli XL1-Blue. Overnight cultures of the transformants were resuspended in final sample buffer and analyzed in an immunoblot with serum (1:5,000) from a rabbit hyperimmunized with formalin-killed, virulent L. kirschneri. The clone number is indicated above each lane (lanes 1 and 4, LipL32; lanes 5, 9, and 29, LipL45; lane 14, unknown; lane 26, GroEL; lane 27, unknown; lane 34, LipL41; lane C, negative control that does not express an antigen). The positions of molecular mass standards (in kilodaltons) are indicated on the left.