FIG. 2.

Sequence of the leptospiral gene encoding LipL45. The nucleotide sequence of clone 5 (accession number AF379683) from position 760 through position 2109 is shown. Probable −35 and −10 promoter sequences are indicated by large letters. The Shine-Dalgarno sequence is underlined. The spirochetal lipobox is indicated by boldface type, and the proposed signal peptidase II processing site is indicated by an arrow. The sequences of the PCR primers used to generate the probe for the Southern blot are indicated by multiple arrowheads. The putative ρ-independent transcriptional terminator is indicated by dashed arrows. The amino acid sequence used to generate serum to the C terminus of LipL45 (5ORF2C) is shaded.