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. 2002 Jan;70(1):323–334. doi: 10.1128/IAI.70.1.323-334.2002

FIG. 4.

FIG. 4.

LipL45 expression in low-passage isolates of various serovars of Leptospira. Leptospira isolates were cultured from the kidneys of infected hamsters and passaged serially in Bovuminar PLM-5 medium up to five times (P2 to P5). Leptospires were resuspended in final sample buffer prior to immunoblot analysis with LipL45 antiserum (1:4,000). Lane 1, L. kirschneri serovar grippotyphosa strain RM52; lane 2, L. interrogans serovar pomona strain P10637-46; lane 3, L. interrogans serovar canicola/portlandvere strain Mex 1; lane 4, L. interrogans serovar canicola/portlandvere strain CDC Nic 1808; lane 5, L. kirschneri serovar grippotyphosa strain ISU 82; lane 6, L. kirschneri serovar grippotyphosa strain RM52, high passage.