LipL45 expression in high-passage pathogenic and saprophytic isolates of various serovars of Leptospira. A panel of leptospiral serovar isolates was examined by immunoblot analysis with LipL45 (1:5,000) and ImpL63 (1:4,000) antisera. Lane 1, L. kirschneri serovar grippotyphosa strain Moskva V; lane 2, L. interrogans serovar pomona strain PO-01; lane 3, L. interrogans serovar bratislava strain AS-05; lane 4, Leptospira noguchii serovar proechymis strain LT 796; lane 5, L. noguchii serovar fort bragg strain Fort Bragg; lane 6, L. kirschneri serovar mozdok strain 5621; lane 7, L. kirschneri serovar grippotyphosa strain RM52; lane 8, Leptospira borgpetersenii serovar hardjo strain HB-15B/93U; lane 9, Leptospira santarosai serovar canalzonae strain CZ 188; lane 10, L. santarosai serovar bakeri strain 79; lane 11, Leptospira wolbachii serovar biflexa strain codice; lane 12, Leptonema illini 3055; lane 13, Leptospira inadai serovar lyme strain 10; lane 14, L. borgpetersenii serovar javanica strain Veldrat Batavia 46; lane 15, L. borgpetersenii serovar tarassovi strain Perepelicin; lane 16, L. interrogans serovar pomona strain RZ11; lane 17, Leptospira weilii serovar celledoni strain Celledoni; lane 18, Leptospira biflexa serovar patoc strain Patoc I; lane 19, Leptospira meyeri serovar semaranga strain Veldrat Semarang 173. The lanes containing the saprophytic species L. wolbachii, L. illini, L. inadai, L. weilii, L. biflexa, and L. meyeri are indicated by asterisks (lanes 11 to 13 and 17 to 19).