FIG. 8.
Localization of LipL45 and P31LipL45. (A) High-passage L. kirschneri was fractionated with Triton X-114, and each fraction was subjected to immunoblot analysis with LipL45 (1:5,000) and LipL36 (1:4,000) antisera. Lane W, whole cells; lane P, insoluble pellet; lane A, aqueous fraction; lane D, detergent fraction. (B) Membrane fractions of virulent L. kirschneri were washed with buffer (lanes 2 and 3), NaCl (lanes 4 and 5), urea (lanes 6 and 7), Na2CO3 (lanes 8 and 9), or Triton X-100 (lanes 10 and 11) for 15 min. After this, the membrane was pelleted by centrifugation, and the pellets (P) and supernatant fluids (S) were subjected to immunoblot analysis with LipL45 and LipL41 antisera. Lane 1 contained unfractionated L. kirschneri (W).