FIG. 9.
Variation of P31LipL45 levels with growth phase. (A) L. interrogans serovar pomona and L. kirschneri serovar grippotyphosa were inoculated into Bovuminar PLM-5 medium at a density of 5 × 105 cells/ml, and the cultures were incubated at 30°C. The number of cells in each culture was determined by dark-field microscopy at different times. (B and C) Samples (1 × 108 cells) obtained from the cultures at different times were examined by immunoblot analysis with LipL45 (1:4,000) and LipL41 (1:8,000) antisera. The strains used were L. interrogans serovar pomona strain P10637-46 (B) and L. kirschneri serovar grippotyphosa strain RM52 (C).