FIG. 2.

The icmTSRQPO-containing plasmid (pGS-Lc32) complements the rib mutants. Contact-dependent hemolysis of sRBC was performed at MOI of 25 for 2 h at 37°C and measured by OD₄₁₅ for the release of hemoglobin. The genes harbored by each plasmid are indicated in the box insert. These data are representative of three different experiments, each done in triplicate, and error bars represent standard deviations.