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The icmT gene is essential for pore-forming activity. Contact-dependent hemolysis of sRBCs was performed using the strain GN229 or AA100kmT transformed by plasmids carrying different subclones of icmTSRQ or by plasmids harboring Kan insertions in icmT. In each experiment, AA100 and GN229 were used as positive and negative controls, respectively. These data are representative of three different experiments, each done in triplicate, and error bars represent standard deviations.
