TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or sequence | Reference or source |
|---|---|---|
| Strains | ||
| L. pneumophila | ||
| AA100 | 3 | |
| GN229 | Kanr | 6 |
| GR159 | Kanr | 6 |
| GL208 | Kanr | 6 |
| GP247 | Kanr | 6 |
| GP263 | Kanr | 6 |
| AA100kmT | AA100; icmT:: Kanr | This study |
| AA100kmS | AA100; icmS:: Kanr | This study |
| φp24 | ΔRtxA | 13, 14 |
| NU259 | 130b; LpsG::Kanr | 30 |
| NU243 | 130b; PilD::Kanr | 28 |
| ribrev1 | This study | |
| ribrev2 | This study | |
| ribrev3 | This study | |
| E. coli DH5α | supE44 ΔlacU169 (ψ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | BRL |
| Plasmids | ||
| pBC SK+ | ColE1 origin, lacZα, Cmr | Stratagene |
| pMW100 | pMMB207αβ; Cmr; icmGCDJB, tphA, and icmF region | 29 |
| pGS-LC-32 | pMMB207αβ; Cmr; icmTSRQPO | 34 |
| pGS-LC-47 | pMMB207αβ; Cmr; icmNMLKEG | 33 |
| pTSRQ | pBC SK+; Cmr; icmTSRQ | This study |
| pTS | pBC SK+; Cmr; icmTS | This study |
| pT | pBC SK+; Cmr; icmT gene | This study |
| pkanT | pBC SK+; Cmr; ; icmT:: Kanr | This study |
| pS | pBC SK+; Cmr; icmS gene | This study |
| pkanS | pBC SK+; Cmr; ; icmS:: Kanr | This study |
| pR | pBC SK+; Cmr; icmR gene | This study |
| pkanR | pBC SK+; Cmr; icmR:: Kanr | This study |
| pQ | pBC SK+; Cmr; icmQ gene | This study |
| pkanQ | pBC SK+; Cmr; icmQ:: Kanr | This study |