TABLE 2.
Primers used in this study
| Primer | Sequencea | Restriction enzyme |
|---|---|---|
| P1 (salIIcmTS) | ACGCGTCGACCACAGTTAAAACTTCAAGCTGAACC | SalI |
| P2 (notIRQ2) | ATAAGAATGCGGCCGCTGCTCAGAGCTATTTTT | NotI |
| P3 | CTAGCTAGCACACTTGCTAATATCTCGCT | NheI |
| P4 | CTAGCTAGCCGTTTGTTATGTATGACGG | NheI |
| P5 | CTAGCTAGCCCAGTGGCTCGTTGCT | NheI |
| P6 | CTAGCTAGCGGTTCAGTTAGATCTGCTAG | NheI |
| P7 | ACGCGTCGACATGAGCTTATTCAAGGGGGC | SalI |
| P8 | ATAAGAATGCGGCCGCGGGAACCAAGAATTAGGGG | NotI |
| P9 | ACGCGTCGACAATGTTTGGTGCTGCTGAATC | SalI |
a
Underlined letters are the sequences of restriction enzymes used for cloning.