FIG. 4.

Expression of iNOS mRNA in the spleen cells stimulated with recombinant PLYs. Normal spleen cells were stimulated for 24 h with PLY471 (25 nM), PLY437 (400 nM), or PLY426 (800 nM), at the concentration optimal for IFN-γ induction for each recombinant protein. Total RNA was extracted and subjected to RT-PCR. PCR products obtained by using specific primer sets for iNOS and β-actin were electrophoresed. The predicted sizes of PCR products for iNOS and β-actin are indicated. Bands in the left lane indicate the positions of DNA size markers. The same experiment was repeated twice, and a similar result was obtained.