Figure 3:

Bi-allelic inactivation of TP53 is associated with progression to high-grade serous carcinoma. (A, B) Case 4. (A) Photomicrographs of the spatially distinct serous borderline tumor (SBT) and high-grade serous carcinoma (HGSC) components involving the ovary.

Immunohistochemical stain for p53 shows heterogeneous expression in SBT (wildtype pattern, with inset showing higher magnification of boxed area) with foci of diffuse overexpression (red arrowheads). Diffuse p53 overexpression is present in the HGSC component. (B) Heatmap of pathogenic mutations in matched tumor samples, and phylogenetic tree based on mutations and LOH events. (C-D) Case 5. (C) Photomicrographs of the primary ovarian micropapillary serous borderline tumor (miSBT), indeterminate-grade serous carcinoma (IGSC) involving the diaphragm, with foci suspicious for high-grade transformation, and subsequent recurrence as high-grade serous carcinoma (HGSC). (D) Heatmap of pathogenic mutations in matched tumor samples, and phylogenetic tree based on mutations and LOH events. SNV - single nucleotide variants; DEL – deletion; INS – insertion; LOH – loss-of-heterozygosity.