FIG. 1.

DNA and amino acid sequence of the pSF151 insertion site at homologous antigen I/II regions within the chromosome of the S. intermedius IB08981 and S. mutans IB10991 isogenic mutants. This region corresponds to the 1,099 to 1,154 amino acid residues in the full-length (1,527-amino-acid) S. mutans MT8148 antigen I/II (accession no. P11657). The amplified fragment used for insertional inactivation was flanked by the Tsp509I restriction sites, as indicated. The forward primer used for sequencing and amplification of the target site is underlined. ✽, Identical bases.