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. 2002 Jan;70(1):380–388. doi: 10.1128/IAI.70.1.380-388.2002

FIG. 3.

FIG. 3.

Histopathological (A to C) and immunohistochemical (D to F) analyses of liver. Histology sections were stained with hematoxylin II and eosin, while specific staining of E. chaffeensis-infected mononuclear cells was achieved using alkaline phosphatase-conjugated secondary antibodies with Fast Red TR/napthol AS-MX as the substrate (Sigma Chemical Co.). (A) Representative inflammatory response at day 8 postinfection. Several inflammatory cells and lesions are present (magnification, ×10; H&E staining). (B) Inflammatory response focus containing neutrophils and an apoptotic body (magnification, ×100; H&E staining). (C) Granulomatous inflammation on day 16 (magnification, ×40, H&E staining). (D) Control mouse liver; there is no immunostaining (magnification, ×100; hematoxylin staining). (E) E. chaffeensis-positive red staining in the cytoplasm of inflammation cells and mononuclear cells in hepatic sinusoids (magnification, ×100; hematoxylin staining). (F) E. chaffeensis-positive red staining in macrophage foci and mononuclear cells in hepatic sinusoids (magnification, ×100; hematoxylin staining).