In vitro urease activity of M6 and mutants. cag designations denote the promoter used to drive recombinant ureB expression. Strains with wild-type urease activity [M6, M6(cag9-ureB), and M6(cag25-ureB)] did not differ significantly from each other or from M6ΔureB(hpn-ureB). The urease activity of strains M6ΔureB(cag1-ureB) and M6ΔureB(cag25-ureB) was significantly less than that of the wild type and was significantly greater than zero. Urease activity of the other strains did not differ significantly from that of urease-negative M6ΔureB (see text). ∗, significantly different from M6, P < 0.05. †, significantly different from M6ΔureB, P < 0.05.