TABLE 1.
H. pylori urease mutants used in this study
| Strain | Analogous strain used for in vitro evaluationa | Genotype or description |
|---|---|---|
| M6 | C57 | Wild type |
| M6ΔureB | 412 | Replacement of the ureB open reading frame with a kanamycin resistance cassette (ΔureB::kan) |
| M6(cagx-ureB) | NAb | M6{hpn::[Φ(Pcagx-ureBcat)]}, wild-type strain M6 with the recombinant cag-ureB fusion integrated 55 bp downstream of the hpn stop codon; x denotes the specific cag promoter region; cag promoters used were cag9 and cag25 |
| M6ΔureB(cagx-ureB) | 585(cagx-ureB) | M6ΔureB{hpn::[Φ(Pcagx-ureBcat)]}, M6ΔureB with the recombinant cag-ureB fusion integrated 55 bp downstream of the hpn stop codon; x denotes the specific cag promoter region; cag genes used were cag-1, -9, -13, -14, -15, -21, and -25 |
| M6ΔureB(hpn-ureB) | 472 | M6ΔureB{hpn::[Φ(hpn-ureBcat)]}, M6ΔureB with the recombinant cag-ureB fusion integrated within the coding region of hpn |
a
Detailed descriptions of the mutant constructs and their characteristics in laboratory-passaged H. pylori strain C57 have been published (20).
b
NA, not applicable.