Fig. 5.

PPARγ mediates the antiinflammatory effect of EETs. BAECs were pretreated with AUDA (1 μM) for 2 h and then incubated with various EETs (1 μM) for 8 h. (B) Cells were cultured as in A, except that GW9662 (1 μM) was included in the indicated experiments during the 8-h incubation. All cells were then stimulated with TNF-α (10 ng/ml) for 30 min. The collected cell lysates were immunoblotted with anti-IκBα antibody. α-Tubulin was used as a loading control.