FIG. 3.

Effect of MBL on the uptake of L. major by PMN. (A) PMN were coincubated with L. major promastigotes at a PMN-to-parasite ratio of 1:5 for 10 min at 37°C in medium supplemented with 20% fresh serum from healthy individuals (containing 2.3 μg of MBL per ml ([serum 1] or 2.8 μg of MBL per ml [serum 2]). (B) PMN were coincubated with L. major promastigotes at a PMN-to-parasite ratio of 1:5 for 10 min at 37°C without MBL or in the presence of 3.3 μg of purified MBL in medium without serum supplementation or in medium supplemented with 20% heat-inactivated FCS (hi-FCS). Data are from one representative experiment of two performed.