FIG. 5.

Effect of L. major on induction of the oxidative burst by PMN. PMN were coincubated with L. major promastigotes at a PMN-to-parasite ratio of 1:5 for 10 min at 37°C in RPMI 1640 medium supplemented with either 20% fresh autologous serum or 20% heat-inactivated serum (hi-serum) or without serum supplementation. The oxidative burst in PMN was assessed by using the fluorogenic substrate dihydrorhodamine 123 and measured by flow cytometry (FL-1). The percentage of burst positive cells is shown in the upper left corner.