Table 1.
Real-time RT–PCR detection of LMP-1 and LMP2A
| Samplea | Transcript | Relative fold induction, as compared to EBNA2-only sampleb |
|---|---|---|
| EBNA2 | LMP-1 | 1.00±0.18 |
| EBNA2+EBNA-LP | LMP-1 | 3.42±0.061 |
| EBNA2+Δ3–152Sp100 | LMP-1 | 3.24±0.058 |
| EBNA2 | LMP2A | 1.00±0.19 |
| EBNA2+EBNA-LP | LMP2A | 0.846±0.20 |
| EBNA2+Δ3–152Sp100 | LMP2A | 1.07±0.083 |
| aRNA from cells transfected with the indicated plasmids was reverse transcribed and the cDNA was amplified by real-time PCR as described in Materials and methods. | ||
| bFold induction was calculated using the AB User Bulletin 2 calibrator method for relative quantitation; relative transcript induction was normalized to actin and then expressed as a ratio to the EBNA2-only sample. Numbers represent an average of duplicate samples and are representative of multiple experiments. | ||