Skip to main content
. 2005 Sep 29;24(20):3624–3634. doi: 10.1038/sj.emboj.7600824

Figure 1.

Figure 1

Homo- and heterointeraction of APP family proteins. (A) Homointeraction of myc- and HA-tagged APP, APLP1, and APLP2. Pairs of myc- and HA-tagged APP (myc-APP and HA-APP), APLP1 (myc-A1 and HA-A1), or APLP2 (myc-A2 and HA-A2) were expressed in COS7 cells. In all, 1/25 of each lysate was used as an input control (DL). HA-tagged APP/APLPs were immunoprecipitated and immunoblotted for myc- and HA-tagged constructs. As controls, cells transfected with the corresponding myc-tagged APP family member or postlysis mixtures (lanes ‘mix') of separately transfected myc- and HA-tagged APP/APLPs were immunoprecipitated. (B) Heterointeraction of myc- and HA-tagged APP, APLP1, and APLP2. Heterotypic pairs of myc- and HA-tagged APP, APLP1, or APLP2 were transfected into COS7 cells as indicated. In all, 1/25 of the each lysate was loaded as input control (DL). Myc-tagged APP/APLPs were immunoprecipitated and immunoblotted for myc- and HA-tagged APP/APLPs. Controls were performed as above.