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. 2005 Sep 29;24(20):3519–3531. doi: 10.1038/sj.emboj.7600825

Figure 2.

Figure 2

C. elegans Nup155 is required for NPC assembly. (A) Control (upper panels) or Nup155-depleted (lower panels) embryos were fixed and analysed using anti-Nup153 antibodies (green in overlay) or mAb414 (red in overlay). Chromatin was visualized with DAPI (blue in overlay). (B) Control (upper panels) or Nup155-depleted (lower panels) embryos were analysed with anti-Nup96 (left two columns, green in overlay) or anti-Nup35 (right two columns, green in overlay) antibodies together with mAb414 and DAPI (red and blue in overlay, respectively). (C) Still images from time-lapse recordings of YFP-Nup107-expressing control embryos (left panels) or Nup155-depleted embryos (right panels). The inset in left panel shows a magnification of YFP-Nup107 localization to the metaphase plate (−0:20). See Supplementary video 6. Scale bars, 10 μm.