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. 2005 Sep 29;24(20):3519–3531. doi: 10.1038/sj.emboj.7600825

Figure 3.

Figure 3

Biochemical characterization of Nup155 in Xenopus egg extract. (A) The cytosolic fraction of Xenopus egg extracts was analysed by Western blotting with preimmune (pi αNup155) or anti-Nup155 sera (αNup155). Molecular weight markers are indicated on the left. (B) Equal amounts of protein of the cytosol (Cyt), total membrane (tMem) and floated membrane (fMem) fractions of Xenopus egg extract were probed for the antigens Nup155, Nup62 and Pom121 on a Western blot. The asterisk in the cytosol lane indicates a crossreactivity of the Pom121 antibody.