TABLE 1.
IgG subclass responses to GST-MSP119 and GST-MSP133 in C57BL/6 and BALB/c mice
| Mouse strain | Immunogen | Mean ELISA titera (103) ± SD
|
|||
|---|---|---|---|---|---|
| IgG1 | IgG2a | IgG2b | IgG3 | ||
| C57BL/6 | GST-MSP119 | 120 ± 29 | 0.18 ± 0.070 | 21 ± 11 | 0.076 ± 0.028 |
| GST-MSP133 | 41 ± 25 | 0.74 ± 0.81 | 56 ± 46 | 0.44 ± 0.36 | |
| BALB/c | GST-MSP119 | 260 ± 160 | 2.8 ± 1.8 | 15 ± 11 | 0.47 ± 0.88 |
| GST-MSP133 | 147 ± 82 | 4.4 ± 2.9 | 25 ± 9.5 | 0.45 ± 0.38 | |
a
For specific detection of antibodies to MSP1 proteins, sera from mice immunized with GST-MSP119 and GST-MSP133 were tested in ELISA for reactivity with GST-free MSP119 and MSP133, respectively.