FIG. 5.

Structure of MSP-2 and location of CD4⁺-T-cell epitopes. The central hypervariable region (cross-hatched) is flanked by conserved N- and C-terminal regions (solid). The positions of the CD4⁺-T-cell epitopes recognized by cattle with at least three different MHC class II haplotypes (3) are indicated by solid lines, and the amino acid positions (numbering based on 11.2 MSP-2 [21]). The sequences of the peptides representing the epitopes are provided for both A. marginale and A. centrale. The tested peptides include the 312-to-341 P12 peptide and 18-, 19-, and 20-mer peptides representing the different sequences of A. marginale and A. centrale. The amino acid substitution is indicated by an underlined boldface letter.