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Detection of photolyase expression and function. Photolyase expression was confirmed by western blots of whole cell lysates from stably transfected XPA cell lines containing empty vector or both photolyases. Blots were probed with antibodies specific for CPD-PL (A) and 6-4PP-PL (B). Photolyase repair of UV induced DNA damage in XPA cells was detected by immuno-dot-blot, using the same antibodies. Representative examples of 6-4PP (C) and CPD (D) lesion studies are shown.
