Figure 5.
CPD mimic, pTpT, does not induce apoptosis in vivo. TUNEL-stained sections from mouse dorsal skin coated with pTpT or pApA, or exposed to a single dose 4 kJ/m2 UVB as a positive control. (A) Green fluorescence indicates 3' DNA breaks, representative of apoptotic cells. (B) Numbers of TUNEL positive cells in skin sections from three separate experiments, consisting of three mice in each experimental group. Apoptotic values indicate the number of TUNEL-positive cells by tissue length. Pictures were taken on a Nikon ECLIPSE TE2000-U microscope under FITC illumination at 400×.