FIG. 4.
Generation of an arcA mutation in S. enterica serovar Enteritidis SE2472. (A) Structures of the wild-type and mutant arcA alleles. The coding region of arcA is represented by an open box, and Kanr is represented by a cross-hatched box. The positions and designations of the primers used to characterize the mutants are shown next to the alleles. (B) Characterization of two arcA mutant clones by PCR. The genomic DNA of wild-type strain SE2472 and mutant clones 6 and 7 were PCR amplified by using the primers shown in panel A. Numbers on the left are molecular sizes in kilobases.