FIG. 6.

ArcA mutant of S. enterica serovar Enteritidis is more susceptible to GSNO and hydrogen peroxide. Cultures of SE2472 (WT), the SE2472 arcA mutant (arcA⁻), and vector pRB3-273C- and plasmid pRB3arcA-transformed arcA mutant SE2472 (arcA⁻[pRB3-273C] and arcA⁻[pRB3arcA], respectively) were grown in the presence of 4 mM GSNO in pH 5.0 M9 minimal medium (A) or in the presence of 5 mM hydorgen peroxide in pH 7.0 LB medium (B). Bacterial concentrations were determined by plating. The values are the concentrations of surviving bacteria after exposure to GSNO (A) or H₂O₂ (B). At least three experiments were performed, and the results of a representative experiment performed in triplicate are shown. The error bars indicate standard deviations.