FIG. 7.

ArcA mutation does not make S. enterica serovar Enteritidis susceptible to heat, detergent, salt, or low pH. Cultures of SE2472 (WT), the SE2472 arcA mutant (arcA⁻), and vector pRB3-273C- and plasmid pRB3arcA-transformed arcA mutant SE2472 (arcA⁻[pRB3-273C] and arcA⁻[pRB3arcA], respectively) were subjected to 52°C (A), 5% SDS (B), 1 M NaCl (C), or pH 4.0 in LB medium (D). Bacterial concentrations were determined by plating. The values are the concentrations of surviving bacteria after exposure to the stress conditions. At least two experiments were performed, and the results of a representative experiment performed in triplicate are shown. The error bars indicate standard deviations.