Fig. 5. Evoked Vm responses across repeated TUS trials.

(a–b) Heatmaps of baseline-normalized Vm across trials for two example neurons showing progressive reduction (a) or increases (b) of evoked transient responses across trials. Each row represents one trial. Colored bars indicate analysis windows: red = transient period (0–250 ms), blue = delayed period (250–1000 ms). (c–d) Trial-by-trial evoked Vm response amplitude during the transient (ci, di) and delayed (cii, dii) periods for the neurons shown in (a) and (b), respectively. The darker lines are linear fits with the corresponding R2 and p-values indicated. (e) Distributions of the linear fit slope values (rate of change across trials) of the transient Vm across all recorded neurons (N = 161). (f) Same as (e), but for the delayed Vm.