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. 2002 Feb;70(2):889–898. doi: 10.1128/IAI.70.2.889-898.2002

TABLE 1.

Effects of various inhibitors on internalization of E. chaffeensisa

Treatmentb No. of ehrlichiae per 100 cells % Infected cells
None 3,209 ± 236 83.5 ± 6.5
Genistein (100 μM) 0 0
Neomycin (10 μM) 18 ± 11 2.0 ± 0.5
Verapamil (100 μM) 0 0
MDC (250 μM) 132 ± 17 9.0 ± 3.3
a

Reagents were added to THP-1 cells 1 h before infection. At 3 h p.i., the cells were centrifuged and treated with 2 mg of pronase E per ml for 3 min to remove bound uninternalized E. chaffeensis, and then the cells were continuously cultured. The numbers of internalized E. chaffeensis organisms were determined by counting the cells 3 days p.i. The values are means ± standard deviations (n = 3).

b

Genistein is an inhibitor of PTK; neomycin is an inhibitor of PLC; verapamil is an inhibitor of calcium influx; and MDC is an inhibitor of TGase.