TABLE 1.
Treatmentb | No. of ehrlichiae per 100 cells | % Infected cells |
---|---|---|
None | 3,209 ± 236 | 83.5 ± 6.5 |
Genistein (100 μM) | 0 | 0 |
Neomycin (10 μM) | 18 ± 11 | 2.0 ± 0.5 |
Verapamil (100 μM) | 0 | 0 |
MDC (250 μM) | 132 ± 17 | 9.0 ± 3.3 |
Reagents were added to THP-1 cells 1 h before infection. At 3 h p.i., the cells were centrifuged and treated with 2 mg of pronase E per ml for 3 min to remove bound uninternalized E. chaffeensis, and then the cells were continuously cultured. The numbers of internalized E. chaffeensis organisms were determined by counting the cells 3 days p.i. The values are means ± standard deviations (n = 3).
Genistein is an inhibitor of PTK; neomycin is an inhibitor of PLC; verapamil is an inhibitor of calcium influx; and MDC is an inhibitor of TGase.