TABLE 2.
Effects of various inhibitors on proliferation of E. chaffeensisa
| Treatmentb | No. of ehrlichiae per 100 cells | % Infected cells |
|---|---|---|
| None | 6,030 ± 400 | 94.1 ± 2.2 |
| Genistein (100 μM) | 90 ± 20 | 7.9 ± 18 |
| Neomycin (10 μM) | 75 ± 25 | 2.5 ± 0.9 |
| U-73122 (10 μM) | 485 ± 105 | 25.7 ± 3.6 |
| TMB-8 (100 μM) | 148 ± 30 | 18.8 ± 2.8 |
| 2-APB (75 μM) | 475 ± 19 | 30.7 ± 4.6 |
| Verapamil (100 μM) | 12 ± 2 | 1.4 ± 0.4 |
| SKF-96365 (60 μM) | 195 ± 60 | 8.6 ± 0.5 |
| MDC (250 μM) | 0 | 0 |
a
Reagents were added to THP-1 cells at 3 h p.i. and were present throughout the incubation period. The THP-1 cells were infected with host-cell-free E. chaffeensis at an MOI of 100, and infectivities were determined 3 days p.i. The values are means ± standard deviations (n = 3).
b
Genistein is an inhibitor of PTK; neomycin and U-73122 are inhibitors of PLC; TMB-8 and 2-APB are inhibitors of intracellular calcium release; verapamil and SKF-96365 are inhibitors of calcium influx; and MDC is an inhibitor of TGase.