Table 1. Enzymatic activity of Dct and tyrosinase, content of melanin and thiol in non-agouti black versus Dct-mutant melanocytes in culture.
Melanin content* | Enzymatic activity‡ | Thiol content§ | |||||||
---|---|---|---|---|---|---|---|---|---|
Phenotype | PTCA-eumelanin | 4-AHP-pheomelanin | Total melanin† | PTCA/total melanin† | Dct | Tyrosinase | Melanin formation | Cysteine | Glutathione |
Non-agouti black | 1280±16 | 26±0.5 | 599 | 2.14 | 23.58±0.82 | 26.20±8.55 | 53.30±3.29 | 0.27 | 2.78 |
Slaty | 53±3 | 130±10 | 81 | 0.66 | 8.36±0.16 | 25.81±1.42 | 61.46±4.77 | 0.27 | 3.07 |
Slaty light | 302±39 | 662±21 | 198 | 1.53 | 0.88±0.02 | 23.45±3.66 | 60.56±2.77 | 0.60 | 0.29 |
* Expressed in ng/106 cells.
† Expressed in terms of A500×1000/106 cells.
‡ Expressed in terms of pmol·(μg of protein)−1·h−1.
§ Expressed in terms of nmol/106 cells.