Table 1. Kinetic parameters of α-glucosidase from F. acidiphilum for hydrolysis of sugar-based substrates.

Activity was measured at 50 °C in 100 mM sodium citrate buffer (pH 3.0).

Substrate	kcat (s−1)	Km (mM)	kcat/Km (s−1·mM−1)
Sucrose [α-D-Glc-(1→2)-α-D-Fru]	94.0±11.0	0.32±0.06	293.0±44.0
Maltose [α-D-Glc-(1→4)-D-Glc]	126.0±8.0	0.64±0.09	197.0±20.0
Maltotriose [α-D-Glc-(1→4)-D-Glc-(1→4)-D-Glc]	13.0±1.1	0.69±0.08	18.8±1.8
Maltotetraose [α-D-Glc-(1→4)-D-Glc-(1→4)-D-Glc-(1→4)-D-Glc]	2.8±0.1	9.30±0.40	0.3±0.1
p-Nitrophenyl α-D-glucopyranoside	22.5±3.5	5.00±0.10	4.5±0.6