Table 1. Kinetic parameters of α-glucosidase from F. acidiphilum for hydrolysis of sugar-based substrates.
Substrate | kcat (s−1) | Km (mM) | kcat/Km (s−1·mM−1) |
---|---|---|---|
Sucrose [α-D-Glc-(1→2)-α-D-Fru] | 94.0±11.0 | 0.32±0.06 | 293.0±44.0 |
Maltose [α-D-Glc-(1→4)-D-Glc] | 126.0±8.0 | 0.64±0.09 | 197.0±20.0 |
Maltotriose [α-D-Glc-(1→4)-D-Glc-(1→4)-D-Glc] | 13.0±1.1 | 0.69±0.08 | 18.8±1.8 |
Maltotetraose [α-D-Glc-(1→4)-D-Glc-(1→4)-D-Glc-(1→4)-D-Glc] | 2.8±0.1 | 9.30±0.40 | 0.3±0.1 |
p-Nitrophenyl α-D-glucopyranoside | 22.5±3.5 | 5.00±0.10 | 4.5±0.6 |