Table 2. Transgene combinations, enzyme activities and lifespans of Dm4SOD/OCAT and Dm4Control flies.
Enzyme activities were determined at 10 days of age in whole body homogenates except for mitochondrial catalase activity, which was determined at 2 days of age. Results are expressed as units/mg of protein and are means±S.D.; n.d., not determined.
| Catalase activity | |||||
|---|---|---|---|---|---|
| Chromosomes | Transgene combinations | MnSOD activity | Total | Mitochondrial | Mean lifespan (days) (n)* |
| Control | Dm4Control | ||||
| Chr. 2/Chr. 2 | c23, c6/c7, c24 (C1) | 39±2 | 283±3 | 7±23 | 77.2 (100) |
| c22, c24/c6, c21 (C2) | 56±2 | 219±11 | 11±9 | 83.8 (100) | |
| c21, c7/c24, c23 (C3) | 45±1 | 257±7 | 2±9 | 86.2 (100) | |
| Chr. 2; Chr. 3 | c23, c6; c3, c10 (C4) | 48±3 | 259±6 | n.d. | 70.7 (98) |
| c22, c24; c2, c5 (C5) | 47±4 | 178±4 | n.d. | 90.6 (99) | |
| c21, c7; c8, c9 (C6) | 54±11 | 226±16 | n.d. | 89.3 (100) | |
| Mean±S.D. | 48±6 | 237±37 | 83±8 (597) | ||
| Experimental | Dm4SOD/OCAT | ||||
| Chr. 2/Chr. 2 | M8, O6/M2, O2 (E1) | 51±3 | 659±28 | 243±19 | 50.9 (99) |
| M9, O5/M7, O7 (E2) | 95±6 | 517±25 | 194±23 | 47.3 (99) | |
| M5, O4/M7, O5 (E3) | 98±4 | 449±20 | 214±5 | 59.8 (100) | |
| Chr. 2; Chr. 3 | M8, O6; M3, O1 (E4) | 100±7 | 588±27 | n.d. | 65.6 (99) |
| M9, O5; M6, O1 (E5) | 78±1 | 568±7 | n.d. | 69.3 (100) | |
| M5, O4; M1, O3 (E6) | 89±6 | 428±37 | n.d. | 66.3 (99) | |
| Mean±S.D. | 85±19 | 538±88 | 60±9 (596) | ||
* n is the number of flies in the lifespan experiment.