Table 3. Mitochondrial function of Dm4_SOD/OCAT and Dm4_Control flies.

Mitochondrial respiration rates (states 3 and 4) were determined using pyruvate+proline as substrates and flies 10–15 days of age. Rates are expressed as nmol O₂·min⁻¹·mg of protein⁻¹, and are means±S.D. for four measurements. The RCR is the ratio of state 3 rate/state 4 rate, and the ADP:O ratio is the number of ADP molecules per oxygen atom during state 3 respiration. Results from a representative experiment are shown; the experiment was repeated independently three times (pooled results are presented in Figure 4). Aconitase activity is expressed as mM β-NADPH·min⁻¹·mg of protein⁻¹ and values are means±S.D. for three independent experiments, each involving two different preparations, with four measurements per preparation.

	Control (C3)	Experimental (E1)
Respiration rate (nmol of O2·min−1·mg of protein−1)
State 3	633.2±13	631.5±44
State 4	58.8±3	71.5±7
RCR	10.8±1	8.9±1
ADP:O ratio	3.1	3.0
Aconitase activity (mM β-NADPH·min−1·mg of protein−1)	0.50±0.07	0.53±0.04