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. 2005 Oct 10;391(Pt 2):301–310. doi: 10.1042/BJ20050523

Figure 5. Purification of Rpn10/p54-interacting proteins by immunoprecipitation.

Figure 5

The cross-linked DEAE-fractogel sample shown in Figure 4(A) (20 min) was immunoprecipitated with a mixture of mAb 28 and mAb 170. mAb developed against potato leghaemoglobin was used for control immunoprecipitation. Aliquots of the precipitated materials were analysed by immunoblotting. Lane 1: the immunoprecipitate obtained using potato leghaemoglobin antibody was analysed with mAb 170. The immunoprecipitate produced with a mixture of mAb 28 and mAb 170 was analysed with mAb 170 (lane 2), mAb IIG7 (lane 3), mAb 50 (lane 4), mAb 112 (lane 5) and mAb 123 (lane 6).